SKU: 70643186423

Rat GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit

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Description

Rat GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA KitThe Rat GP4 CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit measures GP4 CD36 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GP4 CD36 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Rat GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit measures GP4/CD36 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GP4/CD36 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with GP4/CD36 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of GP4/CD36 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 70643186423

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