Pay in installments of $135.38 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 18 - Jul 23
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human Gastric Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm3using ophthalmic scissors or scalpel (4) the tissue was digested with human gastric cancer primary tissue digestive solution C, and then it was shaken at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human gastric cancer organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human gastric cancer organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of human gastric cancer organoid culture medium A. |
||||||||||||||||
| Description | Composition:
|
||||||||||||||||
| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 12 reviews
Sort
Product Reviews
★★★★★ 5
The Best Cable Management Solution for IT Work — Reusable, Reliable, and Professional
Size: 150Pk - 8" x 1/2", Style: Cord Wrap Cable Ties, Color: Black and Gray
TL;DR: I’ve been using these VELCRO Brand cable ties for years as an IT field tech. They hold tight, are endlessly reusable, and keep cables tidy. Great for both home and professional setups.
Full Review:
As an IT field technician, I’ve tried countless ways to keep cables organized, and these VELCRO Brand cable ties are simply the best. They’re reusable, strong, and easy to reposition — ideal for network racks, AV installs, or just cleaning up cords around the house.
Functionality:
Top-notch. The hook-and-loop material is aggressive enough to hold firmly, but not so sticky that it clings to everything else in your tool bag. They wrap cleanly, stay put, and release easily when you need to re-route cables. They’re reliable enough for professional jobs and flexible enough for home use.
Storage Capacity:
Each strap holds more than you’d expect — I rarely need to link two together. They handle thick cable bundles or power cords just fine on their own. And when I do need more length, it’s easy to join a second strap for a larger wrap.
The two-color mix (black and gray) is great for keeping different cable types separated — for instance, network vs. power. The thin profile also helps keep bundles neat and professional-looking.
These have earned me plenty of compliments from higher-ups for clean cable runs and well-organized setups. Once you switch to these, you’ll never want to deal with cutting zip ties again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 12, 2025
★★★★★ 5
VELCRO Ties Are the Standard to Rely On
Size: 150Pk - 8" x 1/2", Style: Cord Wrap Cable Ties, Color: Black and Gray
I am a big fan of VELCRO brand products because of their quality and utility. I have used their rolls of Velcro hook and loop tape as well as their little patches, and I became a brand loyalist. Yes, there are many other manufacturers, but these are certainly as good as it gets. This product is your very basic, very thin, and very copious supply (for my neighbors in Rio Linda - that means plenty of them). You get two big spools of these little eight (8) inch ties that are adhesive throughout their length - that means you can tie the smallest cables to anything almost up to their full length. If you need a longer wrap - use more than one and just extend the length by piggy backing them. The real advantage of this bulk package is that you are not tempted to save some for later - you have enough to use as many as you like and can happily go tie crazy on all your cords! It's VELCRO party time. We live in an electronic age, and I have a lot of more pricey hook-and-loop specialty ties, and I try to keep a few back as spares. Forget that - with these little ties, I can actually use them and do what I need to get those tangled cords in shape. O.K. maybe I am a geek, but I cook my chicken - dead already, and I believe in independent segregated wiring and not tripping over things or spending time untangling messes when I need to move gear. These big rolls will make your life easier - I use them to attach notes to little jars or vials - to tie two gloves together when I am not wearing them - and other uses even cutting them into smaller pieces. That's one of the advantages of these bulk spools - lots of supply. Yes, the individual ties are dinky thin little things, but they hold very well (why you buy genuine VELCRO) and you don't feel stupid using them up on anything you feel like. They are expendable, and that adds to their ultimate utility. Darned useful product. Buy it - use it; enjoy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 17, 2024
★★★★★ 5
Computers, electronics cabling? You NEED these!
Size: 100Pk - 6” x ½”, Style: Cord Wrap Cable Ties, Color: Black
If you do any cabling, be it TV or computer or anything else, these ARE worth there weight in gold. Seriously! They'll keep your cable runs, extension cords, etc in a nice neat easy to work with bundle that you can have out of the way. Adhesion is very good also which is also important. Just the right size for most applications and if you need something large they make them to fit your needs. Cable management has always been a pet peeve of mine and makes a difference that not only shows but makes future work and maintenance much easier. Whenever I see a rat's nest cluster of wires I always know it means shoddy workmanship!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2026
★★★★★ 4
Very Effective, but Not the Instructions
Size: 150Pk - 8" x 1/2", Style: Cord Wrap Cable Ties, Color: Black and Gray
These work very well, but when you remove them from the box, you see two donuts, one grey, one black, of 75 tightly wrapped cable ties each. So far, so good.
First deficiency: The "how to use" pictures---three of them---are tiny light line drawings in 7/16" x 7/16" boxes. If you don't have a 400-power microscope, maybe a magnifying glass will do. Maybe Velcro---a "name" brand that should know better---thinks that the use of these devices is obvious. Well, "obvious" is obvious only when it's obvious, and that can vary from person to person. They should assume nothing.
Second deficiency: Before trying (and, one hopes, succeeding) at using these ties, one must determine exactly now to remove the first tie from the outside of either donut. Obvious? No, not really, because, as it turned out, it requires some effort to remove the first one---the placement and circumstances of which do not at all (not on my set of these, anyway) suggest in any concrete way which one should be taken first. No one wants to tear up a couple of ties just to figure out how not to tear up ties by removing them incorrectly or out of order.
The punishment here is four stars that could have been five.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
★★★★★ 5
Must Have for Cable Management.
Size: 100Pk - 6” x ½”, Style: Cord Wrap Cable Ties, Color: Black
Best Velcro out the. Great for cable management. Super easy to use. Cannot be beat for the price. Incredibly useful.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 29, 2026
recommand products
Beat Escape: Beat Escapebeat Escape - COMPACT DISCS
14.98
Mahler / Tennstedt, Klaus: Mahler: Symphony 1 - COMPACT DISCS
17.99
Mahler / Tennstedt, Klaus: Mahler: Symphony 9 - COMPACT DISCS
27.99
The New Seekers: Together / Farewell Album - COMPACT DISCS
26.00
The Men That Will Not Be Blamed for Nothing: Double Negative - COMPACT DISCS
13.99