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Description
Human vitamin D ELISA KitProduct Specification protein VD Usage Specimen requirements: 1 . The specimen should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in 20 Store, but avoid repeated freezing and thawing 2 . Unable to detect containing NaN3
Product Specification
| protein | VD | ||||||||||||||||||||||||
| Usage | Specimen requirements: 1 . The specimen should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in -20℃ Store, but avoid repeated freezing and thawing 2 . Unable to detect containing NaN3 NaN3 Inhibition of horseradish peroxidase (HRP) activity. Operation steps: 1. Dilution of standard: This kit provides one original standard, which can be diluted in a small test tube according to the following chart.
2. Sample addition: Blank wells (no sample and enzyme labeled reagent are added to the blank control wells, and the other steps are the same), standard wells and sample wells to be tested are set respectively. Accurate spike of standard on enzyme-labeled coated plate 50uL, first add sample diluent to the sample well to be tested 40uL, then add the sample to be tested 10uL (The final dilution of sample was 5 Times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well. 3. Incubation: After plate sealing with plate sealing film 37℃ Incubation 30 Minutes. 4. Solution preparation: will 30 Distilled water for double concentrated washing liquid 30 Time dilution for use 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing liquid, and let stand 30 Discard it in seconds and repeat it 5 Times, pat dry. 6. Enzyme addition: enzyme labeled reagent is added to each well 50uL, except for blank holes. 7. Incubation: Same procedure 3. 8. Washing: Same operation 5. 9. Color development: Add color developer to each well first A 50uL And then adding a color developer B 50uL, gently shake and mix evenly, 37℃ Color development protected from light 10 Minutes. 10. Stop: Add stop solution per well 50uL, terminate the reaction (blue immediately turns yellow at this time). 11. Measurement: zeroing with blank holes, 450nm The absorbance of each well was measured sequentially by wavelength (OD Value). The determination shall be performed after addition of the stop solution 15 Within minutes. Calculation: Taking the concentration of the standard as the abscissa, OD The value is the ordinate, draw a standard curve on the coordinate paper, and according to the sample OD The corresponding concentration is found out from the standard curve; Multiply by the dilution factor; Or use the concentration of the standard substance to OD The linear regression equation of the standard curve is calculated from the value, and the sample OD The value is substituted into the equation, the sample concentration is calculated, and then multiplied by the dilution factor, which is the actual concentration of the sample. |
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| Species Reactivity | Human | ||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich method to determine the level of human vitamin D in specimens. Coating a microwell plate with purified human vitamin D antibody to make a solid phase antibody, adding vitamin D to the microwells coated with monoclonal antibody sequentially, and then combining with HRP-labeled vitamin D antibody to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added with substrate TMB to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and vitamin D in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human vitamin D in the sample was calculated by the standard curve. | ||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||
| Synonym | Human VD ELISA Kit | ||||||||||||||||||||||||
| Composition | Kit composition:
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| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
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| Storage Temp. | Store at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||
| Test Range | 50ug/L -1600ug/L | ||||||||||||||||||||||||
| Applications | It is used to determine the content of vitamin D in human serum, plasma and related fluid samples. |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Covers all my passes
Size: 60 Count (Pack of 1)
I've used several brands of eye wipes and I think I'll stop looking any further after using these.
The wipes are super moist and the largest I've come by.
They are soft thick wipes which I appreciate especially after say cutting grass or any outdoor activities that might attract pollen or dust to my lids. I've used some that are very thin and felt harsh.
My skin feels clean yet moisturized after using them.
I'm totally happy that my lids are not burning and itchy anymore.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Relief for your eyes if work on the computer
Size: 60 Count (Pack of 1)
I have been using these wipes for a long time and have been great for my sensitive eyes. Even though I use blue light glasses and toned my monitors for work my eyes would sting like lemons were put on it. After I use these wipes it cools down my eyes and makes it better again. I really recommend these wipes for anyone that has this issue too because it was hard to drive my car when my eyes acted up but after I use the wipes the drive home from the office is like nothing occurred. These wipes dont sting when you use them like the walgreens version does. It honestly feels like a relief when you use them is the best I can describe it. I keep these in my purse, work bag and office drawer since these are individual wipes.
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Reviewed in the United States on April 18, 2026
★★★★★ 5
The Best Eyelid Wipes!
Size: 60 Count (Pack of 1)
These work great for a gentle cleaning and refreshing of the external areas around the eye by removing dirt, pollen or overnight dryness. Unlike some wipes, the size of these are large enough to do the job while being very portable. The wipes are very soft and biodegradable.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Amazing
Amazing lightweight lotion and price is great. It’s actually moisturizing and have no strong smell
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Feels great.
great moisturizer. I think it would take a while to truly review, but it seems like a solid product I will buy again.
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Reviewed in the United States on November 14, 2025