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Description
Human/Monkey/Mouse/Rat/Rabbit/Porcine/Goat/Bovine/Horse/Donkey /Feline/Canine TGF-β1 OneStep ELISA KitProduct Specification Antigen TGF 1 Immunogen Recombinant Protein Antibody Type Recombinant mAb Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 6. 1% Inter assay: 9. 7% Sample type Cell culture supernatant, Serum; Plasma Assay type Sandwich (quantitative) Sensitivity 10. 58 pg ml Range 15. 63 pg mL 1000 pg mL Recovery Cell culture supernatant: 82% Serum: 107%
Product Specification
| Antigen | TGF-β1 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 6.1%； Inter-assay: 9.7% |
| Sample type | Cell culture supernatant, Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 10.58 pg/ml |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 82% Serum: 107% Plasma: 101% |
| Assay time | 60 minutes |
| Species reactivity | Hu; Mk; Ms; Rt; Rb; Donkey; Goat; Horse; Bovine; Porcine; Feline; Canine |
Background
Human Transforming Growth Factor-beta 1 (TGF-β1) is a highly conserved, disulfide-linked homodimeric cytokine (25 kDa) that plays crucial roles in cell proliferation, differentiation, and immune regulation. It is synthesized as a latent precursor consisting of a signal peptide, latency-associated peptide (LAP), and the mature TGF-β1 domain. The mature bioactive form is released from LAP through proteolytic cleavage or acid treatment (pH ≤ 3.0), which disrupts non-covalent interactions while preserving the dimer's structural integrity via critical disulfide bonds. Human TGF-β1 shares the following identity with other species: Monkey (Macaca spp.): ~99%; Mouse (Mus musculus): ~98%; Rat (Rattus norvegicus): ~98%; Rabbit (Oryctolagus cuniculus): ~97%; Donkey (Equus asinus): ~96%; Goat (Capra hircus): ~95%; Horse (Equus caballus): ~96%. The high homology underscores TGF-β1’s critical role in fundamental biological processes across mammals, enabling translational research using animal models. However, species-specific post-translational modifications or receptor interactions may still influence functional outcomes.
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