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Description
mIHC Dye (TSA700)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 700, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.3 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Cute and funny
I got this as a birthday gift for my boyfriend’s sisters dog, and she loves it! It’s very cute to see her run around with it
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Only “tough” toy my Doberman loves!
Pattern Name: Octopus
Not indestructible, but last a good long while. Definitely worth the money and lasts longer than any other plush toys we’ve had (which is all my dog will play with). It’s for sure my Dobermans favorite, I’ve ordered probably 10 at this point. He gets the stuffing out after a month or two and we open a new one. He’s picky with toys, either destroys them or ignores them, but he never gets bored with his octopus! 🐙 He sneaks one outside once in a while and the neighbor’s dogs steal it. They love it too and thankfully can’t destroy before I retrieve it.
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Reviewed in the United States on December 22, 2025
★★★★★ 5
Great for a Pitball
Pattern Name: Octopus
I have a Pitbull and she absolutely loves this toy. I always wait a while before giving a review. She has had this toy Since the beginning of July and she is still playing with this toy. It does have little holes but it is still going. She loves it. Playing tug of war with her. I also take it and wrap it around her collar and watch her dance around trying to get it out(I don't leave it there long). This a great buy. I also bought and extra one so when this one does finally dies she will have another. The material is strong, The sound is about normal for a squeaky toy.
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Reviewed in the United States on December 30, 2025
★★★★★ 3
Cute, but not tough enough.
Pattern Name: Octopus
This toy is cute, my pug loves it, and I like how the legs are strong but the top of the octopuses head isn't tough enough which is worrisome because thats where the squeaker is. It might work out for a smaller dog though.
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Reviewed in the United States on June 12, 2026
★★★★★ 5
Fun Resilient Chew Toy
Pattern Name: Octopus
I have a boxer/pit mix and she loves this! I bought it 4 months ago and it’s still going strong so I know it’s quality made and perfect for dogs that like to chew and play tug of war. It has been chew resistant for my girl and she loves the little squeak. I just throw it in the washer when I wash her bed. Great value and a safe toy that’s a good size for a medium to large dog.
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Reviewed in the United States on May 4, 2026