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Description
Human CAMP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Cathelicidin Antimicrobial Peptide (CAMP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Cathelicidin Antimicrobial Peptide (CAMP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cathelicidin Antimicrobial Peptide ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cathelicidin antimicrobial peptide (CAMP) is a peptide primarily stored in the lysosomes of macrophages and polymorphonuclear leukocytes (PMNs). The CAMP gene encodes the 18 kDa peptide precursor, CAP-18, which is processed into its active form, LL-37, through extracellular cleavage mediated by proteinase 3. Cathelicidin peptides exhibit dual properties, known as amphibian molecules: one end of the molecule is attracted to water and repelled by fats and proteins, while the other end is attracted to fats and proteins and repelled by water. This peptide has been isolated from many different mammalian species. It is primarily found in neutrophils, monocytes, mast cells, dendritic cells, and macrophages. It has also been found in other cells, including epithelial cells and keratinocytes, following activation by bacteria, viruses, fungi, parasites, or the hormone 1,25-D (the hormonally active form of vitamin D). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 5
Slim and Sleek
Size: Small, Color: Black, Size: Small, Color: Black
This slim fit suit was awesome and great. My son loved it and it went perfectly well with my daughter's dress. The perfect way to twin. If anything my son is 5'3 120 lbs and the pants and coat fit a bit long and just took it to a seamstress to get it better fitted for him. Other than that for the price, value, comfort, and look I highly recommend this and so does my son.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Suited me Well
Size: Small, Color: Black
This 2 Piece Slim Fit Suit Set is hands down the best suit for the price.
I bought it for a formal Christmas work party, and it was such a cheap, easy, and quick way to get exactly what I needed — especially since I hate shopping. Honestly, you would never guess I paid as little as I did. It looks sharp, fits great, and feels much more expensive than it is.
The color was a true black, which I really appreciated. It looked clean and classic — perfect for a formal event. It did arrive a bit wrinkled from the packaging, so I had to iron it initially. But it was very easy to iron, and once I did, it stayed pretty crisp throughout the night. It didn’t wrinkle much at all while wearing it.
It’s also way better than going to a store and paying triple the price. And definitely better than renting — now I actually own it and can wear it again whenever I need it.
The fit is perfect if you’re slim. I’m skinny as a rail, and the slim fit worked exactly how I hoped. The jacket isn’t too tight, not too long, and I can move my arms easily without feeling restricted. The whole suit is comfortable and easy to move around in — not stiff like some formalwear can be. It also has handy pockets for your phone and wallet.
Overall, you can’t go wrong with this suit. Great price, true black color, comfortable fit, and sharp look. Highly recommend.
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Reviewed in the United States on February 22, 2026
★★★★★ 5
Amazing fit and quality for the price
Size: Medium, Color: Red
Gotta say I was 100% impressed w this suit! And for the price!!?? Nice fabric. Great fit. Great quality. Fit right out of the box! No alterations were even needed. My 14 year old son is 6'2 and around 155lbs. Broad shoulders. Slender build. We ordered the medium and it fit just right. You really cant go wrong w this suit even if you need to get some alterations you cannot beat the price.
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Reviewed in the United States on May 9, 2026
★★★★★ 4
Quick Delivery & Surprisingly Good Quality
Size: Large, Color: Light Gray, Size: Large, Color: Light Gray
I needed a suit on short notice and didn’t want to spend a fortune, so I took a chance on this gray suit—and I’m really glad I did. For the price, I wasn’t expecting much, but the quality is honestly impressive. The fabric feels solid and looks sharp, not cheap or shiny like other budget suits I’ve seen.
Shipping was fast—I received it in just a couple of days, which was perfect for my tight timeline. The fit was nearly spot-on right out of the box. The jacket and pants fit well overall, though the sleeves were a bit long for my arms.
If you need a reliable suit fast without spending a lot, this is a solid option. It exceeded my expectations for the cost and turnaround time.
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Reviewed in the United States on July 17, 2025
★★★★★ 5
men slim fit suit
Size: Small, Color: Red
I bought this suit for an 11 year old child for their sneaker ball at school. i order him a size small in men's (he wears a 32 in men pants) this suit fitted him well. the quality of the suit had actually exceeded my expectation. this suit was worth the money and the material. I would definitely order this suit for a young boy for a dance and you don't have to worry about spending a substantial amount of money. the material for the suit was good quality and I washed it in the washer machine.
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Reviewed in the United States on April 16, 2026