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Description
Annexin V-PE/7-AAD apoptosis analysis kitProduct Specification Usage 1. Instrument parameter adjustment 1. Collect 1 106 3106The cells were washed twice by centrifugation with pre cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre cooled 1 Binding Buffer to resuspend, and add the same
Product Specification
| Usage | 1. Instrument parameter adjustment 1. Collect 1 × 106-3×106The cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre-cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes. 5. Add 5 uL of Annexin V-PE or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light. 6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube Compensation of channels. Note:Certain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase. The adherent cells can be cultured on a suitable vessel, such as a sterile glass slide, and stained with suitably modified methods. However, this method has not been tested in our laboratory. 2. Sample testing 1. Induce apoptosis according to the experimental protocol. 2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells include cells in the culture supernatant. Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells. 3. Add 5 uL Annexin V-PE and 10 uL 7-AAD per tube. 4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes. 5. Get on the computer for analysis. |
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| Beilstein | 0 | |||||||||||||||
| Synonym | Annexin V-PE/7-AAD Apoptosis Analysis Kit | |||||||||||||||
| Description |
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V Kit Components:
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| PubChem CID | 0 | |||||||||||||||
| General Notes | 1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic. 2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment. |
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| Storage Temp. | Store at 2-8 °C, do not freeze, shelf life 1 year. |
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4.2 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
Fun
Color: Interactive Dog Ball
Fun!
Second purchase. Solid and rugged for 22 lb dog. He loves it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 21, 2025
★★★★★ 5
Great fun for our dogs!
Style: Original
My dogs can’t get enough of this very noisy ball. We have one outside and one in their playroom. It is very noisy. It is very hard. So, I’d say a room with fine furniture is not the place for it. Ours bang it around pretty good.
Our boxer did split one, biting it. But, she’s a serious chewer! Our other dog carries it around sometimes but hasn’t been able to damage it yet. So, I’d say for most chewers it’s going to last a long time.
It’s a great toy but in most households I’d say basement or outside. Again, it’s hard and did ding a few baseboards.
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Reviewed in the United States on March 27, 2026
★★★★★ 5
Superb!
Style: Original
My dog absolutely loves this ball. It's too big for him to fit all the way in his mouth, so it's held up pretty well and still makes the sounds.
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Reviewed in the United States on May 31, 2026
★★★★★ 4
Fun, but buy a couple of them
Style: Original, Style: Original
She loves them. Great toy, but the product can’t hold up- purchased 4-11-2026
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Reviewed in the United States on April 15, 2026
★★★★★ 5
Durable, Rugged, Dog Approved Fun!!
Style: Original, Style: Original
This thing is durable and worth every penny! I bought this over 5 years ago for my rescue and he STILL loves it. He plays with it near daily and it's got a LOT of love-work scrapes, scratches, and scuffs. But it still works perfectly!! Because he's on the medium side, he can't quite bite down on it. Not sure how it'd hold up to heavy chewers with larger mouths. But he can play with this solo for ages and has SO MUCH fun. We love to listen to his "conversations" as he gets into it with this ball. It's been a winner since the day we took it out of the box. It's also the longest lasting toy we still have! Everything else either wore out, was destroyed, or broke. This battery-free ball has made the cut for us.
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Reviewed in the United States on April 2, 2026
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