Pay in installments of $135.38 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 18 - Jul 23
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Organotypic balb/c mouse normal small intestinal organoid culture KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm (4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time) (5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion (6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
||||||||||||||||
| Synonym | Mouse(Balb/C) Small Intestine Organoid Culture Kit | ||||||||||||||||
| Description | Composition:
|
||||||||||||||||
| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.0 ★★★★★
Based on 13 reviews
Sort
Product Reviews
★★★★★ 5
Beautiful design, perfect quality for seasoned destroyers.
Color: Crinkle Duck (Yellow), Size: Large
Absolutely adorable, excellent quality, I have a couple of monsters whose teeth make any kong toy tremble, I was very concerned whether something as soft and nice would last. So far so good.
Good looking and soft and tough at the same time!.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 12, 2026
★★★★★ 5
Best fun indestructible toy
Color: Crinkle Duck (Yellow), Size: Large
Truly indestructible no matter how much our oittie tries. She has owned it for three months works at destroying every day with no success. She carries it everywhere. Best purchase for chewer, more fun than the popular rubber heavy duty toy. Do not hesitate your pup will thank you for this fun distraction.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 4
Happy with quality & price
Color: Crinkle Duck (Yellow), Size: Large
Lasts longer than most toys for the price
Only bought Kong or Outward Hound for
my 17 lb Lhasa Apso in the past a
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 5
My puppy tore it up in about 5min. She had a ball while it lasted.
Color: Crinkle Duck (Yellow), Size: Large
Cute, not durable
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 5
Monkee-doodle loves that it has a crinkle sound when playing tug-of -war
Color: Crinkle Chicken (Yellow), Size: Large
Monkee-doodle likes this toy because it has a squeaker and it also has a crinkle noise maker inside. She loves playing with the toy and it’s easy to spot since it is bright yellow in color. It is suitable for dogs and doesn’t have any pieces that can come loose and harm your pet.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 1, 2026
recommand products
Warning Tools Builder Plumber Electrician Mens Ringer T-Shirt FotL
10.49
Chef Ask About My Knife Collection Funny Mens Ringer T-Shirt FotL
10.49
A Cthulhu Kraken Octopus Mythology Childrens Kids Hoodie
13.99
Science Like Magic But Real Funny Nerd Geek Mens 80% Cotton Hoodie
20.99
Offensive Pizza Eating Skull Chef Childrens Kids Hoodie
13.99