SKU: 9818138884

Human TNF-α High Sensitivity ELISA Kit

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Description

Human TNF-α High Sensitivity ELISA KitProduct Specification Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte derived; TNFA; TNF A; TNFalpha; TNF alpha; TNF alphacachectin; TNFATNF, macrophage derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor alpha Description Testing Principle This

Product Specification

Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Description

Testing Principle:
This kit uses double antibody sandwich ELISA.Specific anti-human TNF-α Capture Antibody is precoated on a high affinity conjugate plate.  Standard substance, sample to be tested and biotin labeled detection antibody were added successively into the plate well of the enzyme label. After fully shaking and mixing, the samples were placed at room temperature for a 2-hour incubation process. The TNF-α in the samples was bound to the solid phase antibody and detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of color reaction was positively correlated with the concentration of TNF-α in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  


Detection type:Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity:100 μl

Kit composition:pre-coated 96-well plate,Standards,Standard, TNF-α Detector Antibody, Standard Diluent, Detector Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substrate, Washing Solution, Termination Solution, SA-HRP, Sealing Plate Mask and Manual  

Sensitivity:0.16 pg/mL

Detection range:1.56-100 pg/mL

Recovery rate:100-108%

Storage:2-8℃

The standard curve

 

Background:

Tumor necrosis factor α(TNF-α), also known as cachexin and TNFSF1A, is an adipokine that is involved in systemic inflammation and is one of the cytokines that stimulate acute phase responses. It is produced mainly by activated macrophages, but can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It has a variety of regulatory functions in the immune response, but also can be used as a potential pyrogen. In response to stimuli (infectious agents or tissue damage), TNF-α circulates throughout the body, activates neutrophils, alters the properties of vascular endothelial cells, regulates metabolic activity in other tissues, and exhibits tumor-killing activity by inducing local coagulation. TNF-α plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information indicates that TNF-α is also involved in the pathogenesis of AIDS. The determination of TNF-α is also useful for transplantation studies.  

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SKU: 9818138884

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Amazon Customer
Boise, US
★★★★★ 5
My picky dog loves them.
Size: 3.5 Ounce (Pack of 2), Style: Chicken Chips, Size: 3.5 Ounce (Pack of 2), Style: Chicken Chips
I try to give my dog, whom I love so much, the healthies food and treats that i can. She is so very picky and it's difficult to find things she likes. She loves these treats and expectes one every morning. She litterally waites by the pantry door for one. The are healthy Human grate chicken chips. Makes me feel better about giving her a treat. I would Highly recommend.
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Reviewed in the United States on May 13, 2026
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E J W
Alexandria, US
★★★★★ 5
Treats
Size: 3.5 Ounce (Pack of 1), Style: Chicken Chips
Puppy likes them
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Reviewed in the United States on April 21, 2026
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Beatrice Antunes
Alexandria, US
★★★★★ 5
my dog is obsessed with these
Size: 3.5 Ounce (Pack of 1), Style: Chicken Chips
my senior lhasa apso loves these. he smells them and takes it with happiness and excitement! he loves these, can't get enough.
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Reviewed in the United States on April 4, 2026
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MBburg
Charlottesville, US
★★★★★ 1
Choking Hazard, Do Not Buy!!
Size: 3.5 Ounce (Pack of 2), Style: Chicken Chips
These treats are like hard rubber. Neither of my dogs can chew them and end up trying to swallow them, choking every time.
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Reviewed in the United States on March 4, 2026
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Amazon Customer
Draper, US
★★★★★ 5
Doggy potato chips, only chicken instead
Size: 3.5 Ounce (Pack of 1), Style: Chicken Chips
My dog goes crazy over these. They’re like doggy potato chips.
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Reviewed in the United States on March 20, 2026

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