Sealed LC Jar/Lid Kit (1000 ml) - Injection Port

Sealed LC Jar/Lid Kit (1000 ml) - Injection Port*No Liquid Culture Media Included* This 1000 ml jar (regular mouth) has been designed exclusively for liquid mycelium cultures. This is the sealed version with no vents for air exchange. There is a 13. 2 mm self healing injection port on the lid and a magnetic stir bar is included. This kit does not include the liquid culture media and is not shipped sterile. You must provide the liquid media and heat sterilize before beginning the liquid culture.

*No Liquid Culture Media Included*

This 1000 ml jar (regular mouth) has been designed exclusively for liquid mycelium cultures. This is the sealed version with no vents for air exchange. There is a 13.2 mm self-healing injection port on the lid and a magnetic stir bar is included. This kit does not include the liquid culture media and is not shipped sterile. You must provide the liquid media and heat sterilize before beginning the liquid culture.

Included: 1000 ml jar, airtight lid w/ injection port, magnetic stir bar, alcohol wipe, sterile 10 ml syringe, gloves, syringe needle

After Jar is Filled & Sterilized:

Provide 3-5 ml of spore or mycelium syringe. Work in a sterile environment; whether still air box, flow hood or prepared room. Thoroughly clean the injection port with isopropyl alcohol and sterilize your hands and tools. Quickly set up your syringe with the provided needle and keep the needle cover on until just before injecting the jar. Quickly insert the needle into the injection port and dispense your live sample into the liquid culture. Smoothly remove the needle and replace the needle cover (if reusing this needle in the future, it MUST be sterilized with a flame). Finally, wipe the injection port with an alcohol wipe. Keep this culture in a shaded, room temperature spot that is at least 3 feet off the ground. Every couple of days, manually swirl the culture or use the included magnetic stir bar with the appropriate magnetic stir plate. This will help keep the mycelium free-floating and prevent the culture from forming a solid mass. Depending on the growing conditions and species, you should see growth in 3-10 days. Keep your eye out for cloudiness in the first few days. This tends to signal contamination of the culture (though not always). Once mature, a liquid culture can be refrigerated for at least 6 months.

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