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Description
Human IL-12 p70 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IL 12 p70 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL 12 p70 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-12 p70 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate. After incubation, IL-12 p70 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | Human interleukin 12p70 detection kit, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NKSF1, p35 | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Interleukin 12 p70 (IL-12), also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine consisting of a 40kDa (p40) subunit and a 35kDa (p35) subunit. Human p40 may circulate as a monomer, a homodimer, or a complex that binds to p35 to form IL-12 or binds to p19 to form IL-23. Both homodimers p40 and IL-23 can bind to IL-12R as non-signal antagonists. Alternatively, p40 can also bind to Rβ1, activating microglia and macrophages. The IL-12 p40 subunit shares with another heterodimeric cytokine, IL-23, with similar and distinct biological activity from IL-12. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, microglia, CD8 + DCs (mouse cells only), and non-germinal center (CD38-CD44 +) B cells (human cells only). Functional IL-12 was shown to enhance cytotoxic activity and induce interferon gamma (IFN-gamma) production within NK cells, T cells, and dendritic epidermal T cells. IL12 has also been reported to induce IFN-γ production by macrophages. IL-12, together with other members of the IL-12 family, IL-23 and IL-27, promotes the development of CD4+Th1 immune response. In response to infection, IL-27 is first released, promoting the transition from Th0 to Th0/1. IL-12 is subsequently produced, resulting in Th1 effector cells. IL-12 combines with IL-18 to generate Th1 memory cells from effector cells, which are subsequently activated by IL-23. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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4.5 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Cute and funny
I got this as a birthday gift for my boyfriend’s sisters dog, and she loves it! It’s very cute to see her run around with it
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 3, 2026
★★★★★ 5
Only “tough” toy my Doberman loves!
Pattern Name: Octopus
Not indestructible, but last a good long while. Definitely worth the money and lasts longer than any other plush toys we’ve had (which is all my dog will play with). It’s for sure my Dobermans favorite, I’ve ordered probably 10 at this point. He gets the stuffing out after a month or two and we open a new one. He’s picky with toys, either destroys them or ignores them, but he never gets bored with his octopus! 🐙 He sneaks one outside once in a while and the neighbor’s dogs steal it. They love it too and thankfully can’t destroy before I retrieve it.
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Reviewed in the United States on December 22, 2025
★★★★★ 5
Great for a Pitball
Pattern Name: Octopus
I have a Pitbull and she absolutely loves this toy. I always wait a while before giving a review. She has had this toy Since the beginning of July and she is still playing with this toy. It does have little holes but it is still going. She loves it. Playing tug of war with her. I also take it and wrap it around her collar and watch her dance around trying to get it out(I don't leave it there long). This a great buy. I also bought and extra one so when this one does finally dies she will have another. The material is strong, The sound is about normal for a squeaky toy.
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Reviewed in the United States on December 30, 2025
★★★★★ 3
Cute, but not tough enough.
Pattern Name: Octopus
This toy is cute, my pug loves it, and I like how the legs are strong but the top of the octopuses head isn't tough enough which is worrisome because thats where the squeaker is. It might work out for a smaller dog though.
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Reviewed in the United States on June 12, 2026
★★★★★ 5
Fun Resilient Chew Toy
Pattern Name: Octopus
I have a boxer/pit mix and she loves this! I bought it 4 months ago and it’s still going strong so I know it’s quality made and perfect for dogs that like to chew and play tug of war. It has been chew resistant for my girl and she loves the little squeak. I just throw it in the washer when I wash her bed. Great value and a safe toy that’s a good size for a medium to large dog.
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Reviewed in the United States on May 4, 2026