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Description
Organotial Human Normal esophageal organoid Culture KitProduct Specification Description Composition: Component name Specification Human esophagus cancer organoid medium A 100mL Primary culture buffer B 250mL Human esophagus cancer primary tissue digestive juice C 30mL Organoid passage digestive juice D 30mL Tissue preservation solution E 100mL Organoid cryopreservation fluid F 20mL Organoid subculture buffer G 250mL Instructions 1, primary (1) The harvested tissues should be placed in precooled (2 8 C)
Product Specification
| Description |
Composition:
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| Instructions |
1, primary (1) The harvested tissues should be placed in precooled (2-8° C) In the sampling bottle of tissue preservation solution E, the tissue was quickly transferred to a clean laboratory for tissue processing and cell separation, photographed and recorded. (2) Prepare several culture dishes and add primary culture buffer B precooled at 4 ° C for later use. (3) The sampling bottle was disinfected, and the tissue was placed in the Petri dish. After washing three times with primary culture buffer B, the tissue was cut into tissue blocks of about 1-3mm3in volume using ophthalmic scissors or scalpel. (4) The tissues were digested with normal human esophageal primary tissue digestion solution C and shaken at 37℃ for 20-25min (the digestion was observed at any time during the digestion). (5) A small amount of liquid was observed under the microscope. When more single cells or cell clusters below 70um were observed under the microscope, triploid volume primary culture buffer B was added to terminate the digestion. (6) The filtrate was filtered by a sieve with a pore size of 100um, and the filtrate was collected. After enrichment and centrifugation at 300g for 5min, the supernatant was removed, and the primary culture buffer B was added and resuspended for centrifugation. (7) Matrix glue calculation: The volume of collected tissue was observed after step 6, and matrix glue (abs9495) was added 25 times the tissue volume to resuspend the paving plate. (8) For the example of 24-well cell culture plates, 25uL of tissue matrix glue mixture was dispensed to each well for paving (operation at 4 ° C). (9) The paved culture plate was placed in an incubator at 37 ° C for 10-15min to become gelation, and human normal esophageal organoid medium A was added for culture (return to room temperature). 2, Organoid subculture (1) The medium was aspirated by pipetting gun, and 1-2mL of 4 ° C organoid subculture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) a: When the number of organoids was insufficient or the volume was small: the supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for the fourth step. b: When organoids are large in number or size: The supernatant was discarded by centrifugation for 5min, then the organoid passage digestion solution D was added for 2-3min, then the organoid passage culture buffer G was added to terminate the digestion, the mixture was discarded by centrifugation for 5min, then the organoid passage culture buffer G was added and the mixture was resuspended and transferred to a 1.5mL centrifuge tube, and the liquid was discarded by centrifugation at 300g for 5min for step 4. (4) After the organoid was collected, 25uL of Matrigel was added to the 24-well cell culture plate, and 500uL of human normal esophageal organoid medium A was added to the incubator for 10-15min. 3. Organoids were frozen (1) The medium was removed by suction with a pipetting gun, and 1-2 ml of 4℃ organoid passage culture buffer G was added to each well and placed for 2min. (2) The matrix glue was gently blown by a pipetting gun, collected in a 15mL centrifuge tube and allowed to rest for 10min at 4 ° C. (every 6-8 Wells were a group) (3) The supernatant was discarded by centrifugation for 5min, then an appropriate amount of organoid passage culture buffer G was added and resuspended again, and the liquid was discarded by centrifugation at 300g for 5min. (4) Add appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, take 24-well cell culture plate as an example: the density is 2 Wells and freeze 1 tube, each tube volume is 1.4mL. (5) After making the labeling information and cooling, the samples were transferred to liquid nitrogen for long-term storage. 4, organoid resuscitation (1) 10mL organoid subculture buffer G was placed in a 15mL centrifuge tube. (2) The frozen organoid cells were removed from the liquid nitrogen tank and quickly thawed in a 37℃ water bath. (3) During the water bath melting process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely melted within 1-2min. (4) The dissolved organoid cells were quickly transferred to a 15mL centrifuge tube, gently blown with a pipette for 6-8 times, centrifuged at 300g for 5min, and then the supernatant was removed and the organoid cell precipitate was collected. An appropriate amount of organoid passage culture buffer G was added and resuspended into a 1.5mL centrifuge tube and centrifuged at 300g for 5min. (5) Matrigel was resuspended, 25uL of Matrigel per well was spread in A 24-well cell culture plate, placed in an incubator for 10-15min to gel, and 500uL of human normal esophageal organoid medium A was added. |
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| Storage Temp. | Stored at 4 ° C, valid for 3 months; Stored at -20 ° C, valid for 1 year, see label for details. |
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Product Reviews
★★★★★ 5
Sturdy, Strong, and Leakproof
Size: 10 Inch, Unit Count: 100
Come on now, its paper plates, it means i don’t have to do dishes. Their sturdy, leakproof, and has a cute design. Good for parties and everyday use. Holds a lot, inexpensive compared to name brand, i would buy them again.
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Reviewed in the United States on March 30, 2026
★★★★★ 5
Would purchase again.
Size: 10 Inch, Unit Count: 372.0
Paper plates are something you really need to shop around and these from Amazon are well made and priced to sell. They hold up well when we are looking to have a quick bite for lunch or a midday snack.
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Reviewed in the United States on May 9, 2026
★★★★★ 5
Strong, Reliable Plates for Everyday Use
Style: 10 Inch, Size: 100 Count
These Dixie Ultra paper plates are a great upgrade from regular disposable plates. They’re strong and sturdy, so they don’t bend or leak even with heavier or saucy foods. Perfect for everyday meals, cookouts, or quick cleanups when you don’t feel like doing dishes. The 10-inch size is just right for full meals, and the pack lasts a good while. I’ve used them for hot foods without any issues—they hold up really well. Overall, dependable quality and worth paying a little extra compared to cheaper, flimsy options.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Best Plates Ive ever known!
Style: 10 Inch, Size: 172 Count (Pack of 1), Style: 10 Inch, Size: 172 Count (Pack of 1)
If you’re tired of the "double-plate shimmy" where you have to stack two flimsy plates just to survive a barbecue, the Dixie Ultra 10-Inch Plates are a total game-changer. This 172-count bulk pack is the gold standard for anyone who actually likes to pile on the food.
Why They’re Worth It:
True Strength: The "3X Stronger" claim isn't just marketing fluff. These can handle a massive helping of steak, potato salad, and corn on the cob without folding under pressure.
Soak-Proof Shield: They feature a high-quality coating that keeps grease and sauces from leaking through to your lap or your table.
Microwave Safe: Unlike some cheaper alternatives, these don't get soggy or "wilt" when you're reheating leftovers.
Value Size: Getting 172 plates (divided into 4 convenient packs) means you're set for multiple parties, holidays, or just those nights when you really don't want to do the dishes.
Bottom Line: These are the "heavy lifters" of the paper plate world. They’re large enough for a full dinner and tough enough to handle the messiest meals. Highly recommended for anyone who values durability over price-point flimsy alternatives.
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Reviewed in the United States on April 16, 2026
★★★★★ 5
Quality disposable plates
Style: 10 Inch, Size: 172 Count (Pack of 1)
We purchase these again and again for their durability and thickness for our everyday use of disposable dinnerware. True to size and reliable.
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Reviewed in the United States on May 17, 2026