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Description
Human Pancreatic Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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4.0 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 5
Covers all my passes
Size: 60 Count (Pack of 1)
I've used several brands of eye wipes and I think I'll stop looking any further after using these.
The wipes are super moist and the largest I've come by.
They are soft thick wipes which I appreciate especially after say cutting grass or any outdoor activities that might attract pollen or dust to my lids. I've used some that are very thin and felt harsh.
My skin feels clean yet moisturized after using them.
I'm totally happy that my lids are not burning and itchy anymore.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Relief for your eyes if work on the computer
Size: 60 Count (Pack of 1)
I have been using these wipes for a long time and have been great for my sensitive eyes. Even though I use blue light glasses and toned my monitors for work my eyes would sting like lemons were put on it. After I use these wipes it cools down my eyes and makes it better again. I really recommend these wipes for anyone that has this issue too because it was hard to drive my car when my eyes acted up but after I use the wipes the drive home from the office is like nothing occurred. These wipes dont sting when you use them like the walgreens version does. It honestly feels like a relief when you use them is the best I can describe it. I keep these in my purse, work bag and office drawer since these are individual wipes.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 18, 2026
★★★★★ 5
The Best Eyelid Wipes!
Size: 60 Count (Pack of 1)
These work great for a gentle cleaning and refreshing of the external areas around the eye by removing dirt, pollen or overnight dryness. Unlike some wipes, the size of these are large enough to do the job while being very portable. The wipes are very soft and biodegradable.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Amazing
Amazing lightweight lotion and price is great. It’s actually moisturizing and have no strong smell
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Feels great.
great moisturizer. I think it would take a while to truly review, but it seems like a solid product I will buy again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 14, 2025