Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 29 - Aug 3
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human PDGF ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a platelet-derived growth factor (PDGF) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of platelet-derived growth factor (PDGF) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Platelet Derived Growth Factor（PDGF) ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Platelet-derived growth factor (PDGF) is one of many growth factors that regulate cell growth and division. In particular, PDGF plays an important role in angiogenesis, the growth of blood vessels from pre-existing vascular tissue, the mitosis (i.e., proliferation) of mesenchymal cells (such as fibroblasts, osteoblasts, tenocytes, vascular smooth muscle cells, and mesenchymal stem cells), and the chemotaxis (directional migration) of mesenchymal cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 9 reviews
Sort
Product Reviews
★★★★★ 5
Spot on quality
Size: 6-Foot Cable, Style: Rotating Plug - Single Pack
Excellent quality and met the use and reliability standards I've grown to trust from belkin.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 10, 2025
★★★★★ 5
VERY GOOD
Color: Black, Size: 4 ft (Pack of 1), Style: 4 ft
WORKS GREAT
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 5, 2026
★★★★★ 5
Great product! Worth the purchase
Color: White, Size: 2.5', Style: 2.5 ft
I just wanted a surge protected that's cheap and protects from lightning. I got this product since it was the highest rating for the price. I love this product, and it works great. I have all 6 used and it wasn't that hard to plug in the outlets as some say. It is a little harder than usually, but they don't fall out anymore! I haven't had lightning hit yet so I don't know how well it is against it, but it does come with a lifetime warranty! I just had a bad storm and it worked great, but no lightning struck the power line. I love the product for the price just don't like that it doesn't have the protected light on it (to let you know if you are still protected from lightning). Great product! Also this is a little bigger than a normal 6 outlet, but at least this one includes a surge protection!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 3, 2011
★★★★★ 5
Good
Color: White, Size: 10 ft (Pack of 1), Style: 10 ft
Good
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 27, 2026
★★★★★ 4
Better than the basic power strip
Color: White, Size: 2.5', Style: 2.5 ft
I needed to get a basic five or six outlet power strip and instead ended up with this
. Since the power strip that I was going to get was for basic protection, and would be plugged into a surge suppressor anyway, I really wasn't concerned about how much protection it offered. Just having the extra outlets for additional small-to-medium electrical devices was important.
While looking and comparing this with basic power strips, I decided to check the manufacturer's site to see what specs they offered, since the ones here seemed to be a bit inadequate. Here's what I found:
* 555 Joule energy rating provides maximum protection of all your sensitive electronic devices
* 6 Surge-protected outlets supply 2-line AC protection
* 2.5-foot power cord delivers optimal, safe AC power through a 14-gauge heavy-duty cord
* Filters EMI/RFI noise up to 43 dB reduction
* Maximum Spike Amperage of 36,000 Amps
* Lifetime Product Warranty
* $15,000 Connected Equipment Warranty
The pros on this unit include the price, the basic surge protection, a short but sturdy power cord, and good basic construction. It has a power/reset switch that's recessed at the top of the unit near the power cord to prevent accidental shutting down. On the negative side, like so many of these units, the outlets are too close together to put more than a couple transformer-type devices like cell phone chargers and such.
Just a couple of comments on the specifications listed above to those who may be interested: if you're going to plug high-end expensive electronics into one of these, don't do it. This is an adequate but very basic unit, and you want to consider getting a better grade surge suppressor for large screen televisions, good computer systems and such. No surge protector is 100% effective, but sure that the product is listed as a transient voltage surge suppressor, and that it meets the criteria for UL 1449, the absolute minimum performance standard for surge suppressors. I'm no electrical engineer or electronics technician, but if you're going to protect an expensive jogging machine, your new 52-inch television or Apple iMac 27-inch desktop system, you need better than an under $10 surge suppressor or power strip.
A friend who I will refer to as the 'cranky electronic tech' told me to look it up on the 'Net, but make sure that whatever I got for protection of "really good stuff" needed to be minimally have a UL1449 3rd Edition certification and be rated at 1400 joules of surge protection or higher, and the rest would fall into place. The
that I've used for years with my large electronics is rated at 3330 Joules, and meets those specs.
If you plan to use a basic surge suppressor to charge cell phones and the like with their transformer plugs, units with right-angle outlets are a better, though more costly choice. My personal choice for mid-range protection combined with outstanding quality is the
, a 5-star product that I own and have reviewed here. I run two notebook computers with this, along with a few chargers. There are others if you look.
If all that you're looking for is a basic surge protector with a reasonable amount of surge suppression, then this one from Belkin is a decent choice, and certainly better than the basic power strip. I actually came back recently and bought a second one this year. But if your needs call for more, then the above listed alternates may be worth a look.
4/25/2012
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 26, 2012
recommand products
Southerk 24" x 28" Wall Cabinet (Cabinet Only) - Slate Grey 33549-ASB-1WX Kohler
508.02
Essential 20" x 32" Arch Framed Mirror - Moderne Brushed Gold 30638-BGL Kohler
311.25
Essential 24" x 36" Arch Framed Mirror - Brushed Nickel 30637-BNL Kohler
354.75
Tresham Vanity Top With Single Faucet Hole - Ice Grey 2979-1-95 Kohler
372.68
Helst 36" Bathroom Vanity Cabinet With Sink And Single-Hole Quartz Top - White 33526-ASB-0 Kohler
1176.27